http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2015203677-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14430ce0c28e36044927f5834e4d02ad |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81230cf5049b65b05ad04cce7f25dec6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_120d223f37d6893c0f2924f518c3fc54 |
| publicationDate | 2016-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2015203677-B2 |
| titleOfInvention | Detection of Target Nucleic Acid Sequences by PTO Cleavage and Extension |
| abstract | The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target 5 nucleic acid sequence is cleaved to release a fragment and the fragment hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adopted for 10 detection of the presence of the target nucleic acid sequence. WO 2012/096523 PCT/KR2012/000287 Fig. 2 A. Hybridization DNA or 5' a mixture of nucleic acids Upstream primer PTO3 5' L) 3' 5'3' 3' 5' Downstream primer B. Primer extension & Cleavage of PTO Fragment released from PTO (PTO fragment) 5' 3'' 5' 3' 3' 5' C. Hybridization of PTO fragment to CTO PTO fragment 5'f 3' 5'3' 3'1 b5' 3'-i 5' 3 3 35 CTO Hybrid between uncleaved PTO and CTO D. Extension of PTO fragment 5. 3' 5' 3' 3 ' 5' Extended duplex E. Melting analysis 3' 3' 5'/ 3' 5 3'5' |
| priorityDate | 2011-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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