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filingDate 2014-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber AU-2014243145-A8
titleOfInvention Biological assay of peptidoglycans
abstract The present invention relates to a biological method for assaying peptidoglycans (PGN) in a sample, particularly a sample of glucose polymers. The PGN assay includes: a) treating the glucose polymer sample by sonication, heating, and/or alkalizing; b) placing the treated sample or a dilution thereof in contact with a recombinant cell expressing an exogenous TLR2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with the TLR2. The reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of PGN in the sample using a standard curve of the correlation between the amount of PGN and the strength of the reporter gene signal.
priorityDate 2013-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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