http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2013331771-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42a59c5faed1d0699d86edc5a2eee671 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-952 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate | 2013-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfcea18c65dd19fb41973a6d72a679e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c34e5c051c772f6e4c942193ef1cff2e |
| publicationDate | 2018-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2013331771-B2 |
| titleOfInvention | Cellular test systems for the determination of the biological activities of neurotoxin polypeptides |
| abstract | The present invention pertains to a method for the generation of neurotoxin-sensitive, neuronal differentiated cells comprising the steps of: a) cultivating tumor cells which are able to differentiate into neuronal cells in a culture medium under conditions and for a time which primes said tumor cells for neuronal differentiation; and b) cultivating the tumor cells primed for neuronal differentiation of a) in a differentiation medium having an osmolality of 100 to 270 mOsm/kg, and comprising (i) B27 supplement and/or (ii) N2 supplement, for at least 3 days, thereby obtaining neurotoxin-sensitive,neuronal differentiatedcells. The invention further relates to neurotoxin-sensitive, neuronal differentiated cellsobtainable by the method of the invention. In addition, the invention encompasses a method for determining the activity of a neurotoxin polypeptide comprising the steps of: a) contacting the neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention with a neurotoxin polypeptide; b) cultivating the neurotoxin-sensitive, neuronal differentiated cells of step a) for 3 to 74 hours or 72 hours under conditions which allow for the neurotoxin polypeptide to exert its biological activity; and c) determining the activity of the neurotoxin polypeptide in the said cells after cultivation according to step b). Finally, the invention provides for a medium comprising Opti MEM, FBS, B27 supplement, and N2 supplement. |
| priorityDate | 2012-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.