http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2013208951-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4afb8b01aa8de7e0b24a86713b23a18 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
filingDate | 2013-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f8be19d3a2a280fca0f60d7c03adaab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2441df6fcd5ef4633a699baac8d2e44 |
publicationDate | 2018-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | AU-2013208951-B2 |
titleOfInvention | Cellular high throughput encapsulation for screening or selection |
abstract | The invention relates to a method for selecting a sequence set from a library of expressed nucleic acid sequences, wherein cells are provided, each cell comprises an expressed nucleic acid sequence expressed as a target protein. The cells are encapsulated by treating them with a cationic polysaccharide and subsequently treating them with an anionic polysaccharide, yielding encapsulated cells, perforating the membrane of the encapsulated cells, yielding solubilized compartments, contacting them with a ligand to said target protein, the ligand bearing a detectable label, and selecting a subset of solubilized compartments as a function of detectable label and isolating the expressed nucleic acid sequences from the selection as a selected sequence set. |
priorityDate | 2012-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.