http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2012227297-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_20fef70df872bcdea74c1b83a70db07f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2012-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc7bee25e4b00413e033bb6aa6a7a7e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_686a442f52fb1c511189324020af521a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8054b81ed6d74c0c54a092c253d34ff2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_440878abe1f8b95a5ed92cd2c9f7c9e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6fc45a4114989cf9f2ec46756574f83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b978abed439fb818df35bb972feb83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b746689a759f9e970e0e06f9c74574d7 |
| publicationDate | 2013-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2012227297-B2 |
| titleOfInvention | Combinatorial DNA Library for Producing Modified N-Glycans in Lower Eukaryotes |
| abstract | The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained. Man ctl,2-Man cxl,6 2/20 ER Man al,2-Man al,3-.Yna I Man 11,4-GIcNAc P1,4-GlCNAW-lAsn Man a1,2-Man al,2-Man czl,3 IMafl9GIc7Nk2 "Man~a1,2-Mn~aj, a-1,2 man nosidlase Manal3 ---Man al 16\ Man a1,3-' ~/ M an 131 .-G INAc 1 ,4-G IcNAcpIAsn Man oQ1,2-Man al,24vMan al,3 Man 8GlcNAc2 Man ai,6 ~ ral.2 mannosidase (IA. 18. IC) Manal, -- Mn a, .6/Man P1,4-GlcNAc pI1 4-GlcNAcJ31.Asn Man cxl,3 / Man5GlcNWC2 Man al,6 04.(1.2 GnTI Man a1l,3--- Ma )6Man pi1 4-GloNAc P31,4-GIcNAcI3I-Asn GlcNAc 13,2-Man zl ,3~ GlN 3ln cN~c Man al 16 \ 4mannosldase IE GOLGI G~oN~c f31,2 Man 13 01,4..GIcNAc 114-GIcNAcI3I-Asn GLIGIcNAc 31,2-Ma 4,.3 (3. nU.B14G lNT I f-14nT lc GIcNAc 1 ,24 Man al.6\ 12TI014 TZ -,GT GcNAc 031,4 /Man 131,4-GcNAc 131,4-GlcNAcJpl-An GIcNAc P1,2 -Man al,3 Cornex cati Gl 14cNAc 1,2w 1.Ga Gal 131,4-G cNAc P1 2 1,4 a a Gal P1,4-GIcNAc 13,4... \Man p31,4-GlcNAc 13,4-GIcNAcp1-Asn Gal 131,4-GlcNAc 01,2X' NANA Gal 1,4-GIcNAc 1 , 2 N a2,3 ST. 2,ST NANA Gal P 1,4-GlNAc P 1,4 ~,.Man a a 14Gc~ 14Gc~p-s NANA Gal 13,-lNc 13 'M 1 aI3,4-GIcNAc 131,4-GcN P1.As (NANA Gal 13,4-GlcNAc 13,2~ nCmlxGyorti FIG. lB |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015100058-A1 |
| priorityDate | 2003-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1553.