http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2012203030-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c118612392fffc8b67572d61f23406a2 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-46 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-32 |
filingDate | 2012-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_474fa5c04e10812027f36b5ba144e0b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47cd7cde4be496d60208d59a94288af2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca90b4fb16a0a7162f254221f1dc4d91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbd2c95d55d0bccdea9861edec1717e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fb3c0b78e2b36c7fd525b2fc9339712 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f455d2021a650bbbebc66b8413d63dd |
publicationDate | 2015-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | AU-2012203030-B2 |
titleOfInvention | Method for purification of alpha-1-antitrypsin |
abstract | A streamlined method for purifying alpha-I -antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV, precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, 5 such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in 0 high yield on a commercial scale, is also described. |
priorityDate | 2002-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 229.