http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2011242404-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95a9fc42018421eb7be37c6f2a7efbfb |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2011-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4dd9f5bdece9db685c9182c05e7587c |
publicationDate | 2015-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | AU-2011242404-B2 |
titleOfInvention | Tethered enzyme mediated nucleic acid detection |
abstract | The present invention relates to methods and compositions for detecting a target nucleotide sequence in a sample. In particular, the methods and compositions of the present invention employ a reporter enzyme tethered to a solid support via a tether nucleotide sequence. A disclosed method for detecting a target nucleotide sequence comprises providing a linker nucleotide sequence comprising a probe sequence that can hybridize to the target sequence and a trigger which can hybridize to the tether nucleotide sequence when the probe sequence binds to the target. The linker nucleotide can have a stem-loop structure. When the trigger hybridizes to the tether nucleotide sequence, the tether is able to be digested by a nuclease, releasing the reporter enzyme from the solid support. The activity of the released reporter enzyme on a spatially or temporally separated substrate generates a detectable signal. In some embodiments, the reporter enzyme releases a feedback trigger that can hybridize to the tether nucleotide sequence and release further reporter enzymes, amplifying the signal via a feedback loop. |
priorityDate | 2010-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 143.