abstract |
Disclosed is a collagen gene construct which can be easily refined and which maintains a triple helix structure equivalent to that of natural collagen while having a low molecular weight. Specifically, because CR-D, which is a signal peptide, has a sugar chain recognition region, one-step refining is enabled by means of affinity refinement, and by substituting a portion of a human collagen structural gene for the collagen-like structural gene part of MBL, a low molecular weight collagen which maintains a triple helix structure with high purity and in a large amount and which has thermal stability can be obtained. |