abstract |
This invention relates to methods of remodelling a peptide to have a specific glycan structure attached thereto. This includes the cell free in vitro addition and/or deletion of sugars to or from a peptide molecule in such a manner as to provide a glycopeptide molecule having a specific customized or desired glycosylation pattern, wherein the glycopeptide is produced at an industrial scale. A key feature of the invention is to take a peptide produced by any cell type and generate a core glycan structure on the peptide, following which the glycan structure is then remodelled in vitro to generate a glycopeptide having a glycosylation pattern suitable for therapeutic use in a mammal. Using the methods and compositions of the invention peptide molecules can be generated on an industrial scale that have desired and/or modified glycan structures, thereby, for the first time, providing the art with a practical solution for the efficient production of improved therapeutic peptides. |