| abstract |
Disclosed are: a signal peptide for secreting/producing a virus-like particle (VLP) at a high level, which comprises a modified product of a signal sequence derived from a West Nile virus (WNV); an expression vector for secreting a WNV VLP, which comprises a nucleic acid encoding the signal peptide, a nucleic acid encoding prM protein and a nucleic acid encoding E protein; an animal cell line capable of secreting/producing a WNV VLP at a high level, which has the vector introduced therein; a WNV vaccine comprising, as an active ingredient, a WNV VLP produced by using the cell line; and a WNV DNA vaccine comprising, as an active ingredient, the expression vector for secreting the VLP. |