http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AT-509071-A4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3485916c255c78f6d1b6c2b7447e0fd0 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2010-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2011-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AT-509071-A4 |
| titleOfInvention | PROCESS FOR CLEANING MESENCHYMAL STEM CELLS |
| abstract | The present invention relates to a method for purifying and obtaining a mesenchymal stem cell preparation from adipose tissue for spraying tissue comprising the steps of: a) providing fatty tissue, b) separating the fat from excess water, oil and / or blood by centrifugation, c) dividing the fat centrifuged fat from step b) and incubating 30 to 50%, preferably 40%, of the centrifuged fat with collagenase, as well as other connective tissue digesting enzymes, at 30 ° to 45 ° C, preferably 30 ° to 37 ° C, for 10 up to 45 minutes, preferably for 20 to 40 minutes, d) isolating mesenchymal stem cells from the digested fat by centrifugation, e) mixing the isolated stem cells from step d) with the undigested fat from step c), the undigested fat content being 50 % to 70%, preferably 60%. |
| priorityDate | 2010-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 16.