http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-117729-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59e1d955a7b1560dc68cf7fd800e8fac |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ede7adcadd6021cabd2ce1b6249ded3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af226973a2cded1df8049b2cdb9fa5d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0422368122f33bd16eebeaaa8a64eae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_baca39b709ae34197b53587b2b302223 |
| publicationDate | 2021-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-117729-A2 |
| titleOfInvention | ANTIGEN BINDING MOLECULES REPEATEDLY BINDING MULTIPLE ANTIGEN MOLECULES |
| abstract | Antibodies with weaker antigen-binding activity at early endosomal pH than plasma pH have the ability to bind multiple antigen molecules for a single antibody molecule, have long plasma half-lives, and have more time to bind to the antigen. Claim 1: A method for dissociating within a cell an antigen from an extracellularly bound antibody, characterized in that it comprises replacing between one and nine amino acids of the antibody with histidine, or inserting between one and nine histidines in the antibody, wherein an amino acid position of the histidine substitution or histidine insertion is selected from the group consisting of H27, H31, H32, H33, H35, H50, H58, H59, H61, H62, H63, H64, H65, H99, H100b, H102, L24, L27, L28, L32, L53, L54, L56, L90, L92, and L94 according to the Kabat numbering, and the substitution or insertion of histidine increases the KD (pH5.8) / KD (pH7.4) value, defined as the ratio between antigen-binding activity at pH 5.8 and antigen-binding activity at pH 7.4, compared to the KD (pH5.8) / KD (pH7.4) value before histidine substitution or insertion, wherein the antibody binds to an antigen in the human body. Claim 9: A method for producing an antibody, characterized in that it comprises the following steps: (a) substituting between one and nine amino acids of an antibody with histidine, or inserting between one and nine histidines in an antibody, where an amino acid position of the Histidine substitution or histidine insertion is selected from the group consisting of H27, H31, H32, H33, H35, H50, H58, H59, H61, H62, H63, H64, H65, H99, H100b, H102, L24, L27 , L28, L32, L53, L54, L56, L90, L92, and L94 according to the Kabat numbering; (b) determining the antigen-binding activity of the antibody at pH 7.4; (c) determining the antigen-binding activity of the antibody at pH 5.8; (d) selecting the antibody whose antigen-binding activity at pH 7.4 is higher than the activity at pH 5.8; (e) obtaining the gene encoding the antibody selected in step (d); and (f) producing the antibody using the gene obtained in step (e). |
| priorityDate | 2008-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.