http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-117650-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0597f9c318d2aeca86db94711c516383 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-60 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2878 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-39591 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2803 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ae3c1833a57acf5d76d6fedccddbeb3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0304c21482fb98f4bed57eceb1e8ffa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0b1e20b4c0105f44503bd0be32c381b |
| publicationDate | 2021-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-117650-A1 |
| titleOfInvention | POST-PROCESSING OF BISPECIFIC ANTIBODIES CONSTRUCTS |
| abstract | The present provides an improved manufacturing method for the production of bispecific antibody constructs, comprising at least two binding domains. The method comprises at least the method comprising a step of refolding the construct, in which one or more of the domains comprise a disulfide bond, and in which the construct is contacted with a refolding buffer to refold the construct to its native form, the buffer comprising (i) a redox and / or oxidizing agent having a concentration of at least 0.1 mM and (ii) a chaotropic agent having a concentration> 1 M, and in which the pH of the refolding corresponds to +/- 4.5 of the pI value of the first domain or +/- 4.0 of the pI value of the entire construct. Claim 1: A method of purification of a bispecific antibody construct, the construct comprising: a first domain having a pl of 5.0 to 9.5 that binds to a tumor target; a second domain that binds to an extracellular epitope of the human and Macaca CD3e chain; and optionally a third domain comprising two polypeptide monomers, each comprising a hinge, a CH2 domain and a CH3 domain, wherein said two polypeptide monomers are fused to each other via a peptide linker, wherein the construct comprises at least one open disulfide bridge, in which one or more of the domains comprise a disulfide bond, the method characterized in that it comprises a step of refolding the construct, in which the construct is contacted with a refolding buffer to refold the construct in its native form, the buffer (i) comprising a redox and / or oxidizing agent having a concentration of at least 0.1 mM where the redox agent is selected from the group consisting of reduced glutathione / oxidized glutathione, cysteine / cystine, cysteine / cysteamine, cysteine / cystamine, dithiothreitol (DTT), b-mercaptoethanol, and glutathione, and where the oxidizing agent is selected from the group consisting of copper (II), oxygen dissolved ene and (L) -dehydroascorbic acid (DHA), and (ii) a chaotropic agent having a concentration in the range of 1 M to 2.5 M, where the chaotropic agent is selected from the group consisting of urea, dimethyl urea, methylurea, ethylurea, organic solvents and guanidinium, and wherein the pH of the refolding buffer corresponds to +/- 4.5 of the pI value of the first domain or +/- 4.0 of the pI value of the entire construct. |
| priorityDate | 2018-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.