| abstract |
Method for obtaining immobilized enzyme preparations, in particular to the preparation and application of the active enzyme conjugate with a polymeric vehicle. Said conjugate possesses the properties of the drug Longidaza and inhibits hyperplasia of the connective tissue, and anti-inflammatory action, it can also be used to manufacture stable, active and safe drugs in the use of prolonged action in the form of a suppository, an ointment, a injection or a cosmetic cream, and to produce veterinary drugs. This method consists of the conjugation of the hyaluronidase with a water-soluble copolymer that uses the carbodiimide or azide method. The conjugation is carried out with the use of the copolymer of N-oxide poly-1,4-ethylenepiperazone, (N-carboxymethyl) -1,4-ethylenepiperazine or its hydrazide and the 1,4-ethylenepierazine of the general formula (1 ), where n ranges from 40% to 90% of the total number of cells; m ranges from 3% to 40% of the total number of cells; n + m + 1 = 100%. |