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publicationDate 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber AR-091298-A1
titleOfInvention MANIPULATED LEAVES OF YEAST TO PRODUCE ETHANOL FROM ACETIC ACID AND GLYCEROL
abstract Processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, in which genetically modified yeast cells comprising an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with glycerol dehydrogenase activity are used linked to NAD⁺. The process is further characterized in that glycerol is present or is fed to the culture medium, whereby the modified yeast cell ferments hexoses, pentoses, acetic acid and glycerol in ethanol. This is also related to yeast cells that are used in said processes. Yeast cells advantageously comprise genetic modifications that improve the use of glycerol, such as modifications that increase one or more between dihydroxyacetone kinase activity and the transport of glycerol within the cell. Preferably the yeast cell further comprises a functional exogenous xylose isomerase gene and / or functional exogenous genes that give the cell the ability to convert L-arabinose into D-xylulose 5-phosphate and may comprise a genetic modification that increases activity acetyl-CoA synthetase.
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