http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-083569-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7254887bc4324c447a6a7cfda1e6b29c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-85 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-745 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6437 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2011-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf299abdec3ecaa6128a676d2b286a5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b78471105cb13a7f89ee2d34bddafa8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6ca39235523b49be9a56111b7a788eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86ea6b9f9e2f6f6e83fe35bc18ce5e99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d1aa95f4e441378f068268378cf6d25 |
| publicationDate | 2013-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-083569-A1 |
| titleOfInvention | METHOD FOR MASS PRODUCTION OF FACTOR VII / VIIA |
| abstract | A method for the mass production of human coagulation factor VII. The method comprises a) constructing an expression vector containing i) a dihydrofolate reductase promoter devoid of one of more of the CCGCC repeat sequences of the GC-rich region thereof and an operably linked dihydrofolate reductase (DHFR) gene thereto, and ii) a cytomegalovirus (CMV) promoter and a human coagulation factor VII gene operably linked thereto; b) transfect the expression vector of step a) in an animal cell line; c) culturing the transfected animal cell line from step b) in the presence of a dihydrofolate reductase inhibitor to select cells that express human coagulation factor VII with great efficiency; and d) adding sodium butyrate to the animal cells of step c), and a cell line for the mass production of human coagulation factor VII. The use of the vector containing a DHFR promoter devoid of the GC-rich repeat sequences in the method allows the human coagulation factor VII to be produced with great efficiency on a large scale, which contributes to the treatment of hemophilia. An HMF 708 cell line to produce human coagulation factor VII. |
| priorityDate | 2010-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.