http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-079958-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_19dd0d889cedfe1b171584512ae9edaa |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2011-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21b023fe5c35f61a8cdba1a54428020d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a71cb6df5f6aa391a03795df50c7fb96 |
| publicationDate | 2012-02-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-079958-A2 |
| titleOfInvention | HOSPEDADOR-VECTOR SYSTEM FOR THE PROPAGATION OF COIE1 PLASMIDE WITHOUT ANTI-BIOTIC |
| abstract | A host-vector system that uses the copy number control mechanism based on the RNA of ColE1 type plasmids to regulate the expression of a marker gene allows the selection of plasmids without using antibiotics and is useful for the production of plasmid DNA as gene transfer vehicle (eg gene therapy) and for the production of recombinant proteins. Claim 16: A host-vector system comprising a) a plasmid with a ColE1 origin of replication; b) a bacterial host cell in which said plasmid a) can be replicated, which contains i) a DNA sequence encoding a protein whose expression is desired to be regulated and, operatively associated therewith, ii) a DNA sequence encoding a RNA sequence that mimics an RNA II sequence, or parts thereof, and that is complementary to an RNA I sequence that can be transcribed from plasmid a), where said RNA sequence defined in ii), in absence of plasmid a), allows the expression of said protein and where, when said plasmid a) is present within said host cell, the RNA molecule I transcribed from the hybrid plasmid with said RNA sequence defined in ii), with which represses the expression of said protein. |
| priorityDate | 2004-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID426334837 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID46874763 |
Total number of triples: 14.