http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-072563-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_87d22c1ed7acfa7baf9a4d5e0472843a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
| filingDate | 2009-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7ec157193129e05c807a826271bdc4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc68f9ff16e83f3b7cf4fb5e13ee27c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f9735a7e69afa744428dba071e8f6c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cbddb3f546e34ef84af6b1e28211f24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_285c1bcbfd0c63e0c1fd8f49383d118f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_642e78923cf8474e5b07aa696982f9ce |
| publicationDate | 2010-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-072563-A1 |
| titleOfInvention | A PROCEDURE FOR OBTAINING ASPARTIC INSULIN USING A PICHIA PASTORIS LEAVES CEPA |
| abstract | In addition a DNA construct for use in the procedure to obtain aspartic insulin. Claim 1: A method for obtaining aspartic insulin characterized in that it comprises: i) transforming a strain of Pichia pastoris yeasts with at least two expression vectors that have different selection markers from each other and wherein each vector comprises a DNA construct that encodes for an aspartic insulin precursor of formula B (1-30) -X1-Y-X2-A (1-21); ii) select among the transformed strains according to the previous stage, those recombinant strains that are MUT S and that contain more than 5 copies of the DNA construct integrated into the yeast genome; iii) select among the recombinant strains of the previous stage that which has a greater number of copies and that has a higher level of expression of the aspartic insulin precursor; iv) fermenting said recombinant strain; v) capturing the precursor of aspartic insulin from the culture medium by chromatographic techniques, vi) digesting said precursor enzymatically under suitable conditions so that at least 70% of the precursor of aspartic insulin becomes mature aspartic insulin; and vii) purify the aspartic insulin obtained from said digestion by conventional chromatographic techniques. |
| priorityDate | 2009-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 105.