http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-031227-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 |
| filingDate | 2000-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2003-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-031227-A1 |
| titleOfInvention | LISOFOSFOLIPASA, A METHOD FOR THEIR PRODUCTION, A SEQUENCE OF NUCLEIC ACID, A RECOMBINANT EXPRESSION VECTOR, A RECOMBINANT GUEST, PROCESS FOR HYDROLYZING FATTY ACIL GROUPS AND PROCESS TO PERFECT THE FILTRATION SOLUTION CAPACITY OR |
| abstract | Aspergillus lysophospholipases (A. niger and A. oryzae) having molecular masses of approximately 68 kDa and amino acid sequences of 600-604 amino acid residues and a method for their production have been isolated; comprising: (a) a polypeptide encoded by a lysophospholipase coding part of the DNA sequence cloned into a plasmid present in Escherichia coli deposit number DSM 13003, DSM 13004, DSM 13082 or DSM 13083, or (b) a polypeptide having an amino acid sequence such as the mature peptide shown in SEQ ID NO: 2, 4, 6, or 8, or which may be derived therefrom by substitution, deletion and / or insertion of one or more amino acids, in particular by deletion of 25-35 amino acids in terminal C; (c) a polypeptide analog defined in (a) or (b) that: (i) has at least 70% homology with said polypeptide, (ii) is immunologically reactive with an antibody raised against said polypeptide in purified form, or (iii) is an allelic variant of said polypeptide; or (d) a polypeptide that is encoded by a nucleic acid sequence that hydrates under conditions of high severity with a complementary strand of the nucleic acid sequence shown as nucleotides 109-1920 of SEQ ID NO: 1; 115-1914 of SEQ ID NO: 3; 70-1881 of SEQ ID NO: 5; or 193-2001 of SEQ ID NO: 7, or a sub sequence thereof having at least 100 nucleotides. The new lysophospholipases have only limited homology to known amino acid sequences. They also isolated genes that encode the new enzymes and cloned them into E. coli strains. A recombinant expression vector, a recombinant host cell, a process for hydrolyzing fatty acyl groups in a phospholipid and a process for improving the filtration capacity of a suspension or aqueous solution of carbohydrate-containing origin containing phospholipid are further described. |
| priorityDate | 1999-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.