http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-027615-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44367c7b17bf07d2d403f117a3fe1a97 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-19 |
| filingDate | 2001-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2003-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-027615-A1 |
| titleOfInvention | A PROCESS TO PRODUCE A BIOLOGICALLY ACTIVE PURIFIED FREE FORM OF THE RECOMBINANT HUMAN GAMMA INTERFERON |
| abstract | A process for producing a purified, biologically active, free form of recombinant human interferon gamma (HIG) by means of espressioncytoplasmic in Escherichia coli (E. coli), which comprises the steps of: a) growing a culture of E. coli cells inoculated with a plasmid that carries the gene for HIG in a fermenter; b) induce the culture to an OD of 5.0 with an inducer selected from the group consisting of isopropyl-beta-D-thiogalactopyranoside (IPTG), indolacrylic acid (IA A), indolpropionic acid (IPA) and lactose; c) harvest the fermentation broth 5 hours after the induction; d) inactivate the cells in the collection broth; e) concentrate inactivated cells by primary clarification through a microfilter; f) shred the concentrated inactivated cells by homogenization at 600 bar (60 MPa) in three passes; g) centrifuge the homogenatofinal; h) purifying the free form of the recombinant human gamma interferon from the supernatant of step (g) by means of cation exchange chromatography; and i) recovering the purified recombinant human gamma interferon after the final step of dialysis or gel filtration chromatography. |
| priorityDate | 2001-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.