titleOfInvention |
ISOLATED POLINUCLEOTIDE SEQUENCE, RECOMBINANT EXPRESSION CASSETTE, VECTOR, GUEST CELL, TRANSFORMED VEGETABLE CELL, PLANT, SEED, POLYPEPTIDE ISOLATED, METHODS TO REDUCE THE PATHOGENICITY OF A HONGO, THAT PRODUCTS A METROCHAINED METHODRING |
abstract |
The present invention provides polynucleotides and related polypeptides of the APAO enzyme isolated from Exophiala. In addition, APAO enzyme encoding polynucleotides can be used to transform normally susceptible plant cells to Fusarium or other toxin-producing fungal infection. Plants can be regenerated from transformed plant cells. In addition, the present invention provides the expression of both APAO enzymes and a steroidum esterase in a transgenic plant. In this way, a transgenic plant can be produced. In this way, a transgenic plant with the ability to degrade fumonisin can be produced, as well as with the ability to produce degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in prokaryotic systems as well as in non-plant eukaryotic systems. Methods for detoxification of grains, grains in processing, silage, food crops and rumen microbes and animal feed are also described. |