http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AR-011848-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_170c8ebd815eccf6cd88533c412db263 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_880cc8535f892eca8121f608c316808c |
| filingDate | 1998-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2000-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AR-011848-A1 |
| titleOfInvention | THE EUCARIOT CELL LINE USEFUL IN THE IDENTIFICATION AND QUANTIFICATION OF INHIBITORS OF THE BETA-AMYLOID PROCESSING ROUTE, DNA MOLECULES FOR THE DESIGN OF SUCH CELL LINE, IDENTIFICATION METHODS, HIGH PERFORMANCE AND COMPOSITION |
| abstract | A eukaryotic cell line having an exogenous gene construct, the exogenous gene construct of which consists of a sequence coding for a cytomegalovirus promoter, a sequence coding for a ribosome binding site, a sequence coding for B-amyloid precursor protein, a sequence encoding of a selectable marker and a coding sequence of a polyadenylation signal. Said seukaryotic cell line is capable of producing more than 30 ng of 4.2kDa beta amyloid protein molecule per mg protein. For the design of cell lines, a substantially purified nucleic acid molecule is used. Eukaryotic cell lines are useful in a method to identify an inhibitor of the beta-amyloid processing pathway, consisting of: administering the inhibitor to said eukaryotic cell line and quantifying a protein molecule indicative of inhibition of the beta-amyloid processing pathway. They are also useful in a method of identifying a beta-amyloid processing pathway inhibitor, consisting of (a) incubating a beta-amyloid processing inhibitor and a eukaryotic cell line to produce a protein molecule that is indicative of the inhibition of the beta amyloid processing pathway in a medium containing a labeled amino acid and (b) separating the eukaryotic cell line and the medium; (c) separating the protein molecule from the aukaryotic cell line and the medium, and (d) quantifying the protein molecule. Another method is a high throughput assay for the identification of a beta-amyloid processing inhibitor, consisting of (a) incubating a beta-amyloid processing inhibitor and a eukaryotic cell line to produce a protein molecule. |
| priorityDate | 1997-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6547 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID414859283 |
Total number of triples: 11.