title |
Competitive antagonist activity at human adenosine A1 receptor expressed in Flp-In-CHO cell membrane assessed as rightward shift in NECA concentration response curve of inhibition of cAMP accumulation without affecting maximal effect at 1 uM preincubated for 10 min followed by forskolin stimulation and further incubated for 30 mins in presence of NECA by alphascreen LANCE assay |