title |
Inhibition of wild type recombinant HIV-1 subtype B BH10 p66/p51 reverse transcriptase heterodimer expressed in Escherichia coli BL21 (DE3) assessed as inhibition of dTTP incorporation on poly(rA)-oligo(dT) template primer duplex preincubated with enzyme for 2 mins followed by incubation with poly(A)-oligo(dT) templates for 2 hrs by flourescence assay method |