title |
Inhibition of Escherichia coli Stx2 in human HeLa cells assessed as stimulation of protein synthesis by measuring increase in [14C]-leucine incorporation at 30 uM incubated with cells for 4 hrs prior to Stx2 addition and further incubated for 20 hrs and subsequent [14C]-leucine addition measured after 6 hrs by microbeta liquid scintillation counting analysis |