title |
Inhibition of recombinant human CYP2J2 expressed in baculovirus infected insect cells co-expressing NADPH-CYP reductase and cytochrome b5 assessed as reduction in CYP2J2-mediated EPA epoxidation by measuring sum of EEQ and DiHETEs level at 1 to 50 uM using EPA as substrate preincubated for 5 mins followed by NADPH addition and measured after 15 mins by LCMS/MS analysis |