title |
Antagonist activity at NMDA receptor in human SH-SY5Y cells assessed as reduction in NMDA/glycine-stimulated calcium influx at 10 uM preincubated for 30 mins in presence of Mag-Fura-2/AM dye followed by NMDA/glycine stimulation and measured over 35 secs by fluorescence assay (Rvb = 100%) |