title |
Inhibition of influenza A virus (A/Mississippi/03/01 (H1N1)) neuraminidase activity using 4-MUNANA as substrate assessed as reactivation half life by measuring reaction rate to reach 50% of maximum rate of uninhibited control after removal of inhibitor preincubated for 30 mins followed by compound washout and subsequent substrate addition measured after each 10 mins for 4 hrs by fluorescence assay (Rvb = 15.6 +/- 6.3 min) |