title |
Poison activity at recombinant human topoisomerase-2alpha expressed in Saccharomyces cerevisiae JEL1 harboring topoisomerase1 deletion mutant assessed as concentration required to triple the level of DNA cleavage complex relative to basal level using supercoiled pBR322 DNA as substrate measured after 6 mins by ethidium bromide staining based agarose gel electrophoresis |