title |
Poison activity at recombinant human topoisomerase-2beta expressed in Saccharomyces cerevisiae assessed as stabilization of DNA-enzyme cleavage complex by measuring concentration required to generate 10% double-stranded DNA breaks using supercoiled pBR322 DNA as substrate incubated for 6 mins measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis method |