title |
Binding affinity to hemoglobin (unknown origin) assessed as increase in release of carbon monooxide from carboxyhemoglobin by measuring half life of carboxyhemoglobin at 20:1 compound/Hb tetramer ratio incubated for 1 hr measured every 15 mins for 90 mins (Rvb = 80 +/- 0.3 min) |