title |
Activation of CaMKK2-phosphorylated AMPK10 alpha2/beta2/gamma1 (unknown origin) assessed as ratio of 5-(6-chloro-5-(1-methyl-1H-indol-5-yl)-1H-benzo[d]imidazol-2-yloxy)-2-methylbenzoic acid EC50 to test compound EC50 using fluorescein-labeled SAMS peptide as substrate pretreated for 30 mins in presence of AMP followed by substrate addition measured after 60 mins by fluorescence assay |