title |
Inhibition of human recombinant topoisomerase 1B expressed in topoisomerase 1B- deficient Saccharomyces cerevisiae EKY3 assessed as decrease in relaxation of negatively supercoiled pSK DNA at 100 uM preincubated for 5 mins followed by supercoiled pSK DNA addition measured after 5 mins by aggarose gel electrophoresis |