title |
Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssRNA binding to Caenorhabditis elegans GST-tagged CRN-4 expressed in Escherichia coli BL21 (DE3) pLysS at 1 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay |